anti-st2 mab  (R&D Systems)

Journal: Tropical Medicine and Infectious Disease

Article Title: IL-33 Enhances the Total Production of IgG, IgG1, and IgG3 in Angiostrongylus cantonensis -Infected Mice

doi: 10.3390/tropicalmed9050111

Figure Lengend Snippet: Levels of total IgG ( A ) and IgG1 ( B ) in the sera of mice. N: non-infected mice. Ac: A. cantonensis -infected mice. +IL-33: the mice received injections of IL-33. +anti-IL-33 or +anti-ST2: the mice received injections of anti-IL-33 mAb or anti-ST2 mAb. The injections began at 3 dpi and subsequent booster injections were administered at 5-day intervals with the same dose.

Article Snippet: The dose of anti-IL-33 mAb and anti-ST2 mAb is 10 µg in 0.1 mL of PBS (R&D Systems Inc., USA) and 50 µg in 0.1 mL of PBS (R&D Systems Inc., USA), respectively.

Techniques: Infection

Journal: Tropical Medicine and Infectious Disease

Article Title: IL-33 Enhances the Total Production of IgG, IgG1, and IgG3 in Angiostrongylus cantonensis -Infected Mice

doi: 10.3390/tropicalmed9050111

Figure Lengend Snippet: Levels of IgG2a ( A ), IgG2b ( B ), IgG2c ( C ), and IgG3 ( D ) in the sera of mice. N: non-infected mice. Ac: A. cantonensis -infected mice. +IL-33: the mice received injections of IL-33. +anti-IL-33 or +anti-ST2: the mice received injections of anti-IL-33 mAb or anti-ST2 mAb. The injections began at 3 dpi and subsequent booster injections were administered at 5-day intervals with the same dose.

Article Snippet: The dose of anti-IL-33 mAb and anti-ST2 mAb is 10 µg in 0.1 mL of PBS (R&D Systems Inc., USA) and 50 µg in 0.1 mL of PBS (R&D Systems Inc., USA), respectively.

Techniques: Infection

Journal: Tropical Medicine and Infectious Disease

Article Title: IL-33 Enhances the Total Production of IgG, IgG1, and IgG3 in Angiostrongylus cantonensis -Infected Mice

doi: 10.3390/tropicalmed9050111

Figure Lengend Snippet: Levels of IgE in the sera of mice. N: non-infected mice. Ac: A. cantonensis -infected mice. +IL-33: the mice received injections of IL-33. +anti-IL-33 or +anti-ST2: the mice received injections of anti-IL-33 mAb or anti-ST2 mAb. The injections began at 3 dpi and subsequent booster injections were administered at 5-day intervals with the same dose.

Article Snippet: The dose of anti-IL-33 mAb and anti-ST2 mAb is 10 µg in 0.1 mL of PBS (R&D Systems Inc., USA) and 50 µg in 0.1 mL of PBS (R&D Systems Inc., USA), respectively.

Techniques: Infection

Journal: Tropical Medicine and Infectious Disease

Article Title: IL-33 Enhances the Total Production of IgG, IgG1, and IgG3 in Angiostrongylus cantonensis -Infected Mice

doi: 10.3390/tropicalmed9050111

Figure Lengend Snippet: The proportion of each IgG subclass in the sum of IgG1 (1), IgG2 subtypes (2a, 2b, and 2c), and IgG3 (3) in each group. The blood samples were collected at the 3rd week post-infection. N: non-infected mice. Ac: A. cantonensis -infected mice. +IL-33: the mice received injections of IL-33. +anti-IL-33 or +anti-ST2: the mice received injections of anti-IL-33 mAb or anti-ST2 mAb. The injections began at 3 dpi and subsequent booster injections were administered at 5-day intervals with the same dose.

Article Snippet: The dose of anti-IL-33 mAb and anti-ST2 mAb is 10 µg in 0.1 mL of PBS (R&D Systems Inc., USA) and 50 µg in 0.1 mL of PBS (R&D Systems Inc., USA), respectively.

Techniques: Infection

Journal: Tropical Medicine and Infectious Disease

Article Title: IL-33 Enhances the Total Production of IgG, IgG1, and IgG3 in Angiostrongylus cantonensis -Infected Mice

doi: 10.3390/tropicalmed9050111

Figure Lengend Snippet: The proportion of each IgG subclass in the sum of IgG1 (1), IgG2 subtypes (2a, 2b, and 2c), and IgG3 (3) in each group. The blood samples were collected at the 4th week post-infection. N: non-infected mice. Ac: A. cantonensis -infected mice. +IL-33: the mice received injections of IL-33. +anti-IL-33 or +anti-ST2: the mice received injections of anti-IL-33 mAb or anti-ST2 mAb. The injections began at 3 dpi and subsequent booster injections were administered at 5-day intervals with the same dose.

Article Snippet: The dose of anti-IL-33 mAb and anti-ST2 mAb is 10 µg in 0.1 mL of PBS (R&D Systems Inc., USA) and 50 µg in 0.1 mL of PBS (R&D Systems Inc., USA), respectively.

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: Increased Dysfunctional and Plastic Regulatory T Cells in Idiopathic Orbital Inflammation

doi: 10.3389/fimmu.2021.634847

Figure Lengend Snippet: IL-33 restored the suppressive function of Tregs, inhibited the production of interferon (IFN)-γ by regulatory T cells (Tregs) and the activation of orbital fibroblasts (OFs) in idiopathic orbital inflammation (IOI). (A) The plasma level of interleukin (IL)-33 in healthy controls (n = 18) and patients with IOI (n = 22) was assessed by Luminex technology. (B) The proportion of circulating ST2 + Foxp3 + Tregs in healthy controls (n = 14) and patients with IOI (n = 8). (C) IL-33 and (D) IL1RL1 mRNA expression in orbital fat from healthy subjects (n = 4) and patients with IOI (n = 15). (E) Intracellular cytokines secreted by FACS-sorted Tregs from IOI patients (n = 5) were detected after stimulation with IL-33 in the presence of anti-CD3 and anti-CD28 and IL-2. (F) Proliferation of CFSE labeled naïve T cells cocultured with sorted-Tregs under different treatment conditions. (G) Expression of a-SMA in OFs cocultured with Tregs under different treatment conditions. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

Article Snippet: In some experiments of CFSE assay and cytokine measurement, Tregs were stimulated with recombinant human (rh)IL-33 (20 ng/mL) (R&D Systems) and human anti-ST2 mAb (R&D Systems), which competitively bound to the IL-33 receptor (ST2).

Techniques: Activation Assay, Clinical Proteomics, Luminex, Expressing, Labeling